Composite

Part:BBa_M36523:Design

Designed by: Scanner Cheung, Emily Marx   Group: Stanford BIOE44 - S11   (2013-10-29)


ALDH2 actuator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1581
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 40
    Illegal PstI site found at 1581
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1333
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1581
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1581
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 534
    Illegal NgoMIV site found at 1047
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 453
    Illegal SapI.rc site found at 950


Design Notes

Removed ATG at end of BCD because GOI began with ATG


Source

See subparts; BCD=Mutalik, GOI=Vega, terminator=registry

References